Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---NTESTLNGCLVTLRQRTNGGP--ANHPVK--SEVRCIRLNQSVHVGGHLITVGE-HCYDVTASNLPANWYP 1XAK Chain:A ((-1-67)) ELYHYQECVRGTTVILKEPCPSGTYEGNSPFHPLADNKFALTCTSTHF-AFACADGTRHTYQLRARSV------

General information: TITO was launched using: RESULT: Template: 1XAK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 194 -1603 -8.26 -27.17 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -8.26 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.168

(partial model without unconserved sides chains): PDB file : Tito_1XAK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1xak-query.scw PDB file : Tito_Scwrl_1XAK.pdb: