Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------EVRPITDFRLNAFNKRVPGCEFGIFYKLLDDGGTL-IGTQVERVGRSKYSWKFVPYEFAGCKWDKECNPIDK-K---------------------------------------------------------------------------- 4G6Q Chain:A ((6-182)) TTGQMPATSSLVDLLHHPLRWRITQLLIGRSLTTRELAELLPDVATTTLYRQVGILVKAGVLMVTAEHQVRGAVERTYTLNTQAGDADHDGVDADRLRTMFTVFVAGVGGHLDQYLEREQIDPLADGIAFRQTALNLSDEELAEFLTAFGEFLAPYVAHSPAPDRTRRVLSTILIPD

General information: TITO was launched using: RESULT: Template: 4G6Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 193 -268 -1.39 -3.72 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -1.39 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_4G6Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4g6q-query.scw PDB file : Tito_Scwrl_4G6Q.pdb: