Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence QDLRRPLLCDVYIEY----PSGQVFDQALGHDIVPAGETAEISGFKCKTVRGTCVILPTCDTPPEYRVTS----- 4HTC Chain:I ((1-65)) ------------ITYTDCTESGQNLCLCEGSNVCGKGNKCILGGNQCVTGEGTPK--PESHNNGDFEEIPEEYLQ

General information: TITO was launched using: RESULT: Template: 4HTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 206 14706 71.39 282.81 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain I : 0.66 3D Compatibility (PKB) : 71.39 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_4HTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4htc-query.scw PDB file : Tito_Scwrl_4HTC.pdb: