Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFDNPDRSQNWSKTGKIADSSDLLPRFPELQRQPCQITSLGSAGGLSGGAIWRIECDRRLYALRRWPAETT--SARL-RYVHSLQRLWRESNLKFIPELYTTAEGESFTENALGFWELAEWMPGAALEGDTISPQQRDAVAAAIAAIHQAA-AQHESSLEPSPGLRQRLTMLQRWRTMDRTMLQDAIQRLGWPEFAAIAQQMLQSFAYAANTIGDELQAIVSTPLPLHACLRDIHREHIFLTG--ALVSGVLDFGAVRIESRAGDLARLCGSLFE---------D--D-R-NRWDDFLARYER--LRPLSAAERRAIAVFDRSAVLLTGLQWIEWIALERRQFPDPAAVLSRLDISLRRLQFLLK 4OCV Chain:A ((24-333)) ---------------TNEALFDVASHFALEG-T--VDSIEPYGDGHIN-TTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKE-TLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFH--DTPHRFEDFKKALAADELGR-----A-AGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLE---------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1286 -28572 -22.22 -98.87 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -22.22 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: