Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVEVNSEETVPEVLLLDLGGITFPSPIGPLLAAAESIRDPGLPRRMVSSVLWKELECGFLTIPEFCEQFAKGKAVVQEALIGWFEGLRAMEPSPRMIILLSELRLKYPGLKIGAVTNNFKHPAPLRPMRDLRGELLFDTIVESAVEGMAKGHPGGERWALV-EVWNFHDGSYMEAHIIWSSVR---------LFKTALERLDYHG-PASAVLYLDDLKHNLAQPASLGIRTRHAITPEE--ATETIRKMLALDVSSGSPLQKYLASLGVLPDSISDQARPFAWQFSFGQSNPTYLVGLIPEKPLEGYTLQKLLEEKCAVLRKQPPGELLPSAHD---VVREYTVMSELGKEGCVPVPRTLGLCTD-KTICGAAFYVMRFVTGVVFTDPNLPN------LQPYQR----------------------RKVYEDLMKTAAAVHNFPWHRSEALTQRFRGDP--SKYVSKQVERWSTQGIDVEGFKRLAKGLLDNVKISQKSPEFQSLVHGDFRLDNCIYDPHTFEVVAVLDWELSTTGNS--LVDIASALSQY--YGLYPGIAQLSSNFEELGIPKERELLDLYLRYREVESIPEGLMQYMEAFQC--------LRMAGILYGVLMRTKKGNASQQHQQAKGGSIFSMAYVTKLCTRGLELLQSRACSSSPIAVDAPSKL-------------------------------------- 4M59 Chain:A ((6-714)) ---------PDAQVLVLAIS----SHPLPTLAAFLASRRDELLRADITSLLKALELSGHWEWALALLRWAGKEGAADASALEMVVRALGREGQHDAVCALLDETPLP-PGSRLDVRAYTTVLHALSRAGRYERALELFAELRRQGVA------PTLVTYNVVLDVYGRMGRSWPRIVALLDEMRAAGVEPDGFTASTVIAACSRDGLVDEAVAFFEDLKARGHAPSVVTYNALLQVFGKAGNYTEALRVLGEMEQNGCQPDAVTYNELAGTEEAARCLDT-MASPNAFTYNTVMTAYGNV-GKVDEALALFDQMKKTGFVPNVNTYNLVLGMLGKKSRFTVMLEMLGEMSRSGCTPNRVTWNTMLAVSGKRGMEDYVTRVLEGMRSSGVELSRDTYNTLIAAYGRCGSRTNAFKMYNEMTSAGFTPCITTYNALLNVLSRQGDWSTAQSIVSKMRTKGFKPNEQSYSLLLQCYAKGG-NVAGIAAIENEVYGSGAVFPSWVILRTLVIANFK-------CRRLDGMETAFQEVKARGYNPDLVIFNSMLSIYAKNGMYSKATEVFDSIKRSGLSPDLITYNSLMDMYAKCSESWEAEKILNQLKCSQTMKPDVVSYNTVINGFCKQGLVKEAQRVLSEMVADGMAPCAVTYHTLVGGYSSLEMFSEAREVIGYMVQHGLKPMELTYRRVVESYCRAFEEARGFLSEVKALEAYIEDA

General information: TITO was launched using: RESULT: Template: 4M59.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2698 -90839 -33.67 -153.96 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -33.67 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.208

(partial model without unconserved sides chains): PDB file : Tito_4M59.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4m59-query.scw PDB file : Tito_Scwrl_4M59.pdb: