Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MVETAYAEPPLAAWSSILTAPRAQRMAHLNPDVRLWSVTADDRSYIVRSF--GRWRPGAGMVDEYRVLLHLKDAGFPVAVPIV--TDAGTLAARDGHRTYVLVPRLAAEKQEELPTGDVCFQIGSAIGNLHAALAQYPWP----VRSYEHDVLQQ--AFDEALQKLPQAVRESSVRPHQA----------VVTSSLR--GLPRQLIHGDCNAGNILVSR--GRVSGVIDL-------------DQLRAAMLAA-------------EITLTSWSHVLLTERRDR--ARPDERARYDAGVAALSWISRHFAEL--------------NAALA---------------------------- 4OCV Chain:A ((24-378)) TNEALFDVASHFALEGTVDSIEPYGDG----HIN---TTYLVTTDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEIDGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELG-RAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREAELLPFSGNLLTMECGMRFLADYLEGDVYFATKYPEHNLVRSRTQIKLVREMEQRADETRAIVADVMETT

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1050 23347 22.23 90.84 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 22.23 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: