Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYTLGQKIGEGGCSEVFEVGD---NRIIKLAKENTSFEALRREYLNNCIAWECGLPIPQPFELTKMSGRPGIVFEHIAGKTMMERFFNQVIVSHNNEVKQADVQLTAQLLFRVHQALAEGKELP--------CQKSIIKSNILSVNYL----SASEKESAISLLES---LETKQCLCHGDPNPRNVIVKDD--GEAILLDWMNATIGNPEADIAEYIVMIRYAVLPSSFPEETRDMFDSIREDLIDIFIDEYYRLSGISYDDVVPWIIPILARKLSADAISDVEKKKLVAEIRRDLGKQKNRV 4H05 Chain:B ((17-251)) ----WVVVEDGASGAGVYRLRGGGRELFVKVAALGA-GVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARW--------PREQRLDVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDP------------WFGPECSAAFLREYGRGWDGA--------------------------------------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 912 -10990 -12.05 -51.11 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -12.05 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: