Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MADSARYTFNMAGI-IMHPGEKRVLLRAVQGGWMLPQVEWQAD---EYSSWKEVARFNREMQGLLDVCLS-----------------------TVRCL----GEDFERATMHFSRIYVMELHSSYDNLPADACWIDIEDLKEFMITPEEHLVW-----LEEWLAKRDEEERTPW-----YHPDWLAKAKVW----------------------IEQALAEPEAQPL--------VSIEQVG----------VAERG---------------CLLRVQTKENAFYFKALPAFFRHELTLLQHLAR----YRVGCVPALVAVDEREHRLLMHDFGSK-------HLD----QSLDLADWQEALRRYAILQIDHIQIDHIQIDHSAQVEQLEELGCP-------RLPLATLPDQLERLLT--------------------------SEQSLL-FDTPTALSLEQV---RRLHDALPLIRSLCQELAGYGLPETLEHG------DLHAQNIALSGGDYLYFDWTDSCIAHPFFSLYPFLLDIEARWPEARIRLREAYLQP-WER--YAARAQLVEAFELAQRLAPLHLAMLYATLIIPHMKARWEMQH--------GVPLNLKVLLDRLHDAG----------------------------- 5TXE Chain:A ((35-686)) APPVTPEVLVRLADIGTMSASETTPLLSLSPDGRYVAFQVRQADPVTNLNVFRMVVKATDGATDAIDVDVGGEYLFWTIPSWGYARNAPSGANLTIQPRWSPSGTHLAYLRQDQGRVRVWRASVKGEG--ASPVIEDAYDIEDVQWLDDNTLIYSGRPGFVEAEAEIEREGRRGWVYDERFHPLTGARPRVLEPISIVYQVLDLKTGTRRAATPTEVARLREKPDPLRAMVGRTTFSVSRTDPQNINAPTTLVARRGEGEPVRCDEEACQNITRMWGDETANVLYFLRREGWASNEMALYRMPADALKPVRIWHATGLLQGCERQAKRLICAQESALQPRRLVTLNLTSGQMSPLYDPNPDLSRYRL-----PKVERLTLRNRNGIEVFSDLVLPPDYQLGTRLPLVIVQYSSRGFLRGGTGDENPILPLATAGFAVLSFHSPRSEASYQRFTSPIAQSKAEYSNWRNRWNILHTLEDLIDDLDRRGVIDPARVGLTGLADGATTVHFGLINSHRFAAAVTSSCCTDSFTASVMNGPRISGALKAYGIETDQADDGPFWAATSFVVNASRLDTPLLIQSADEEYLGALPGFTALQQARKPVELIIYPNEHHVKWQPAHRLAVYNRTIDWFRFWLMDQSDPAPDKAAQYDRWRALRALRQ

General information: TITO was launched using: RESULT: Template: 5TXE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2310 -5975 -2.59 -13.07 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -2.59 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.156

(partial model without unconserved sides chains): PDB file : Tito_5TXE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5txe-query.scw PDB file : Tito_Scwrl_5TXE.pdb: