Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence VCRALIPE-LQDADDSDFDVCRINGGITNISDGRFLSFAVAKVSKKD---------QSVVVRVFGPATEG-VIDRDREIQATCHLSRAGFCPELKGVFKNGIIQTFVTARTLTPEDFLDDAVVAKVAKELRRLHQQEVPGEKES-MVWTEINRYFELASAVTKFESPEDQRKLEAVSFDELRQEINTLKEIGARLKGPVVYAHNDLLPGNVMVDAQG----DKCYYFIDFEYSGYNYRGFDIGTHFNEYAGFDC---------DFCAYPSKDRQLNFLRHYLRPDDPEK---A----THEELEELFVEANFYALAAHITWSAWAIVQATSSAAIDFDYMSYFFKRMKMYREQKAKFLPVVRGFLAGKSK 5FUT Chain:A ((16-376)) -CKEFLPGAWRGLREDEFHISVIRGGLSNML---------FQCSLPDTTATLGDEPRKVLLRLYG-----GAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIK-FTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLP-AFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKIS-SIEFGYMDYAQARFDAYFHQKRKL-------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1258 3064 2.44 10.01 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 2.44 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: