Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKSPDLPVLPGSDQVAPASAIWSSETWRWEAVRWMDTALARRGLERVPTTPLQPRVRPWSTVLVAETTDQGRVWFKAT---SPEMSPEA------AILRALRSVTPDA-----------------------VPVVWADDPERGWLLMPDQGPVLRDVQRDDDAVSLMSSVVRRYARLQRSSTRVVDQLRDAGVPDMSPRESARRWQRLGLKPDTTREVRRAAQRLDAVGLPLTIQHDDLHAGNVFADGTSAGAHDARIFDWGDASVGNPLCSLLVPLERIGEGLDDDAARAARER---ILRAYLSVWSDVVSSTALSAAVDDALLIARVGRVFTWQRALTRASADERHAWGVHGRRLIAEINRSLGHAPEPSHD----- 4ZMU Chain:A ((5-345)) MLACPLPPDEALRQQALDDMALVDTP-----AEHYLD-ALVELARETFGVK----------TVLIS-LIDHDRQWFKARIGLDAEQTPRDLSFCGHAILASEPLMVTDASRDPRFHDNPLVTGPPFIRFYAGEPLHASNGQAIGTLCLIDPSPRLLD-LREGRQLNRLSILAEGYLQL-RSLTEHTRFLRQE--IDREQRKSL-------LDPLTQLWNRAGFHALHQHELELARASDQ-RIGIIYSDID----HFKRINDTLGHRAGDSVLREAASRLRAALRPEDLLARFGGEEFVAMVRVRETTELTMIANRIRELMEATPIDCAGTSVPVTISAGCTLAGSGEE------PERALARADAALYDAKRAGRNRVVSV

General information: TITO was launched using: RESULT: Template: 4ZMU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1537 20642 13.43 68.58 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 13.43 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_4ZMU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4zmu-query.scw PDB file : Tito_Scwrl_4ZMU.pdb: