Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MAAFDVLARLGVDPPDLELLRFGENALYAFRERRLVVRV-ARPSTDPTHVAQTVEFVRRVAARGMPVSEPADLTELVQP---VVTSTGVVTLWTLHDVDEGCR-VSAAELGGLVRQFHELAAPEADLLDEWEPFGLIRS-RLAAAERDGFDRSLTGPLVDLLASLETAVDRLSARLGIG---VMHGDMHYGNVLCLPGR----RLLLIDFDQVCRGPREWDLVPNLVTV-----RRFGLA-ESDYQEFCAAYGFDLRTSPDVETLVRL-RELGMVSWLLQQYGTSAVIDDEIRLRVGTIGESVGPPATRWWAH------------------- 3CSV Chain:A ((4-333)) SREDEIRDFLATHGYADWNRTPRY----QRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDISA--PEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLD----PETLSEMTRLAFS-EYRYAILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIE-FVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1274 -26635 -20.91 -94.79 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -20.91 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.286

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: