Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVRIPDELNAQRRLGPDWENWLDRLPRLCDEIVSDWGLTVVG--EPT-NGFASIVIPVSTSDGDAMLKVGFDSS-PETEHEHLALTYWAGDGA--VTMFRAD--------PARHAMLLEQLHSRDLSQEWDLQACEIVAGFYSRLHRPAPP-RLR-PLTGFVDRWLGALEADAREM-PVPRRLIDHALNRGRAFVD--DPASVGRIIHGDLHYENVLAADREPWLVIDPQPM-SGDPHYELAPMLWNRWDEMD-GYLRESIQRRFYTLVEAAGLD---DDRARDWVIVRMILNAHWAVEDAKRMNRALDADERDWITRCIAVAKAVQR 2PPQ Chain:A ((8-281)) ------------------------TEDELRNFLTQYDVGSLTSYKGIAE---NSNFLLHTTKDPLILTLYEK--KNDLPFFLGLMQHLAAKGLSCPLPLPRKDGELLGELSGRPAALISFLEGMWLRKPE-AKHCREVGKALAAMHLASEGFEIKRPNALSVDGWKVLWDKSEERADEVEKGLREEIRPEIDYLAAHWPKDLPAGVIHADLFQDNVFFLGDELSGLIDFYFACNDLLAYDVSICLNAWCFEKDGAYNVTKGKALLEGYQSVRPLSEAELEALPLLSRGSALRFFLTRL------------------------------

General information: TITO was launched using: RESULT: Template: 2PPQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1042 886 0.85 3.63 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 0.85 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_2PPQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ppq-query.scw PDB file : Tito_Scwrl_2PPQ.pdb: