Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MASAARPIRDYLPGLLVLAGGLYTVYYYYM--YWIRVYGALCLPGYGDPV-KVEVTQ-GIWIRLDAILFPTVFGVILA-----------------LLGVMLVWKKKFGAAVVALVLSATVINWPAFIMIAGGALL--------------GLLFWRKTLLPTIDLPDET--MEWVDAEAGLSVLKSFRRLPS------ERLAYSFHNEGGGRFVVKETSSAESAERETRVLNAAAENGLPAPEAKATDGRFLLMPQLDGFPFLQPL--DFEAWTDEIAGILTTTHSAPKPDMKARRRPAEPVIPDRAF-----DRANWERAARIFREETADAVPVFVHGDFEPANVLFSGGRATG-LTGWDHAGSGPAQL-DVGRMRVALELIHGGNVADRFLAKYKNLSKDRSFRYSRYWDLLAVYGW-LEQDPGSIASHWRRFG--------------ITTLSEDEVMRRLERFVKTLTDKE----- 4TQT Chain:A ((14-490)) ASKVIKGGTVITADRTFRADILIEDGKIAAIGDSLEGDEVIDASGCYVMPGGIDPHTHLQMPFMGTYSSDD---FDTGTAAALAGGTTMVVDFVLPDSEGNLLDALQEWFQKAGKARTDYSFHMAITGWNERTFNEMAEVVKRGINTFHFMAYKGALMVNDDEMFASFQRCAELGAMPLVHAENGDIVAQLQAKLMAEGNDGPEAHAYSRPPEVEGEATNRAIMIADQAGVPLYVVHVSCEQSHEAIRRARQKGM-----RVFGEPLIQHLTLDESEYHNRDWDYAARRVMSPPFRDKLNQDSLWAGLAAGSLQCVATDHCAFTTEQKRYGIGNFTKIPNGTGGLEERMPVLWTRGVRTGRLTPNEFVAVTSTNIAKILNIYPQKGAVVPGADADLVIWDPETTKKISAKTQHSSIDYNVFEGFELKGLPIMTLSRGRIAFDKGQVTAKPGDGRFIEREPNGAVNRALSQWKEIVAPRKVERSA

General information: TITO was launched using: RESULT: Template: 4TQT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2372 32920 13.88 82.71 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 13.88 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.089

(partial model without unconserved sides chains): PDB file : Tito_4TQT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4tqt-query.scw PDB file : Tito_Scwrl_4TQT.pdb: