Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTSSQLNSLRSLPC-FE----KVVDISLLIDGMSHTCIKVTTAL----------QVFFVKKLNSET-----AETEVLSSKYAAKHGLSPRIIYHDNAWLVTDYIVGITLDKNE-LSSSQCIDTGLTLMAKLHQLVPQPNAKTIPCLDTSESVRRLFTNPNSIPSQQ--KLFL---ADIT---ETLTLKIGIEQ---KRSDVTNIICHGDINYSNIIIDETRQSWLIDFECSHLAPVDFDLSMFIAINNI------------------SPLHIDEIVNRYMSLVP----SYK----PNKILLTYYLLYSYFINGLWYLDNTNDAK--INNHLRSLAIEQWSAFDSFVVKYHLDIPKLMPLIS 3C5I Chain:D ((13-355)) --DPLYIKKICLEKVPEWNHFTEDNLRVKQIL--LTNQLFEVGLKEETANNYNSIRTRVLFRIYGKHVDELYNTISEFEVYKTMSKYKIAPQLLNTFNGGRIEEWLYGDPLRIDDLKNPTILIGIANVL-GKFHTLSRKRHLPEHWDRTP-CIFKMMEKWKNQLFKYKNIEKYNCDIHKYIKESDKFIKFMKV-YSKSDNLANTIVFCHNDLQENNIINTN-KCLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLDKVVPTPKLIDEILEAVEVQALGAHLLWGFWSIIRGYQTKSYNEFDFFLYAEQRLKMYDDQ----------------

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1095 -20485 -18.71 -73.42 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.62 3D Compatibility (PKB) : -18.71 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: