Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTIKNDNATAHIILDGRIDSTNAPLAEKQIFEGIHAGTEK-VIIDAENLEFISSAGLRVILKLRKTYKNLEIVGVSPEVYDVFEM-TGFTEMLNIQKGYRKLSVDG-CEVLGEGSNGIVYRYD----PEIVVKVYRNANALNEIER-ERRLARTALILGIPTAIPFDVVRVGNSYGSVFELLNAKSFSKLILAEPEKKDKYIAEFVDLLKRIHSTEVNPEDMEDMKAVAGGAKAVAVGWAEFLRDYLPKEQSDKLLSLIRAVPERHTMIHGDYHTNNVEMQNGEVLLIDMDTLSYGHPVFELASMFLGFVGFGELDHTVTEKFMKLPYETTLYIWHKSLSLYLGTDDEARMKEVAEKAMVVGYARLMRRTIKRIGFDDPAGKKVIDNCKNHLSELLAKVDTLDF 1TQM Chain:A ((1-282)) --------------MNIAELYGKMGKHSWRIMDAIFKNLWDYEYVPLQLISSHARIGEEKARNILKYLSDLRVVQNRQKDYEGSTFTFIGLSLYSLHRLVRSGKVDAIGKLMGEGKESAVFNCYSEKFGECVVKFHKVKVKEHFSVLAIRSARNEFRALQKLQGLAVPKVYAWEGNAVLMELIDAKELYRV---RVENPDEVLDMILEEVAKFYHRG---------------------------------------------------IVHGDLSQYNVLVSEEGIWIIDFPQSVE-------------------VGEEGWREILERDVRNIITYFSRTYRT--EKDINSAIDRILQE-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TQM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1290 12401 9.61 47.51 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 9.61 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_1TQM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1tqm-query.scw PDB file : Tito_Scwrl_1TQM.pdb: