Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQAIPWRDRSFRERFERWIEANVPNATWHVIKDTDLTYL----LVGALNEERYYAKAVTDISRGE-AGLSAFLAERHPDFVP-------DVIAIDSARNWLLMRD----IGGEALRERPDVRRY-EAALRQYAQLQRQEIDQIETYLSYGIPDRRPATLKDEIQTYLPELCAGLDRHQAEAM---LALQDELLTMCDELATGMPMSLEHGDLHGGNIFWRERTNDLCILDWGDATVTHPFFSVRVFWNALYDLLPEEDEVAWYKQIQTMRTVYLEAWSGVAPKDVLWRHLLIAEELGCV----YRALSWHVYVTRYRYDVAESSDKPAQWLNFLLEYRDLKR---RFP---- 3GLF Chain:E ((1-334)) MRWY-----PWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRS--RCRLHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALA-LFQGDNWQARETLCQALAYSVPSGD-----------WYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL

General information: TITO was launched using: RESULT: Template: 3GLF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1377 -42408 -30.80 -139.96 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain E : 0.67 3D Compatibility (PKB) : -30.80 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_3GLF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3glf-query.scw PDB file : Tito_Scwrl_3GLF.pdb: