Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESTDEPGDTVDTELFTRVVRHGLPDVTV--------------ETVTPGGPSWNPVTEVARV------------GFADRDPVYCKAAPGDHEGDLRAEAGTLDYVGATLRVRV--------PSVVRVTTE-------PVAALLTEPVAGRAVADEWFETTPERRATLAERLGRTLATVH--TERFDRAGEITGGDPDGLALDHAPWPEVLRAGVTETQRRAPTDRFDPECARLLDAIDDSRDRLADPPARLLHRDPATPNCFDTGDDRLTLLDWGNS---VVGDPVCDLVRAR---------EQVLAPSREPAPDRLVAPLRRGY-RAVAGGVPSAADR-----------EPVYEAAITLSTAGFVDRLAEW-REESVAELTAWFRAELDGRLSAVE 5L3X Chain:B ((1-405)) GEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQITLALGTAASYPRACQALGAMLSKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQDHKHKYIHILAYAASVVETWKK-NKRVSINKDELKSTSKAVETVHNLCCNENA-----SELVAELSTLYQCI--RFPVVAMGVLKWVDWTVSEPRYFQLQPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSALDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIADPVTEFIAHCKSNFIMVN

General information: TITO was launched using: RESULT: Template: 5L3X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1277 2664 2.09 8.30 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 2.09 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.185

(partial model without unconserved sides chains): PDB file : Tito_5L3X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5l3x-query.scw PDB file : Tito_Scwrl_5L3X.pdb: