Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------HFCEVSVS-YENANKQKITT--------ATKYVVPPGSVDIAVGSQMYAVTVDETCKRTDSIS-IPG---------RFKIDTQGK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 5IC7 Chain:A ((58-422)) RKLRPETIDIQRTRDIPDTHKAAISSLAFHPRYPILLSSSTSSIMYLHKLDASAYPTPNPLLTSVHVKRTDLRRAAFVGPDGGEIIFAGRRRYFHCWNLSSGLVKKVSKIQGHQKEQRTMERFRVSPCGRYMALVASDKKGGGMLNIINVGTMQWIAQARIDGRHGVADFAWWSDGNGLTIAGRDGQVTEWSMITRRTVGIWRDEGTVMALGGRNGPAELGGDRWVAIGSNSGILNVYDRNDLIEIRIKKYPTPTRVFEQLTTSISVVAFSPDGQLLAFGSQHKKDALRLVHLPSCTVYRNWPTEQTPLGRVTAIAFSSKSDVLAVGNDVGRVRLWEIRG

General information: TITO was launched using: RESULT: Template: 5IC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 186 6392 34.37 96.85 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 34.37 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_5IC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ic7-query.scw PDB file : Tito_Scwrl_5IC7.pdb: