Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence SNDKCFVSSYKFTPDGRKVIEASQAAPGDGPITFTSTRDNFHMTIQIDANCAPRNPDDIQKELP 5E24 Chain:B ((1-38)) ------GGRLQFFKDGKFILELARSKDGDKSGWVSVTRKTFRPP--------------------

General information: TITO was launched using: RESULT: Template: 5E24.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 61 1744 28.58 45.88 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 28.58 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_5E24.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5e24-query.scw PDB file : Tito_Scwrl_5E24.pdb: