Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------KCNISILKDGKYFTQKQ-EIAGWTVKFHDLD----GNYVGKCKNAH--TYACTYVTCEDLAPGYSPG 1BIH Chain:A ((307-395)) SAPKYEQKPEKVIVVKQGQDVTIPCKVTGLPAPNVVWSHNAKPLSGGRATVTDSGLVIKGVKNGDKGYYGCRATNEHGDKYFETLVQVN---------

General information: TITO was launched using: RESULT: Template: 1BIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 83 -2448 -29.49 -47.99 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -29.49 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_1BIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1bih-query.scw PDB file : Tito_Scwrl_1BIH.pdb: