Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---GECHITIWEGNTPIGTEIAIEGREYVTIAGYQ-CNAGAGCTAHCIGLPKNLKERGVYH--------------------------------------------------------------------------------------------------------------------------- 4QGL Chain:A ((1-174)) GAAAMAQIRIHEVNTRIENEVKVSKFLQEEGVLYEKWNISK--------LPPHLNEN--YSLTDENKAEILAVFSKEIADVSARRGYKAHDVISLSNSTPNLDELLINFQKEHHHTDDEVRFIVSGHGIFAIEGKDGTFFDVELEPGDLISVPENARHYFTLQDDRQVVAIRIFVTTEGWVPIY

General information: TITO was launched using: RESULT: Template: 4QGL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 68 1285 18.89 27.33 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 18.89 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.56 QMean score : -0.011

(partial model without unconserved sides chains): PDB file : Tito_4QGL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4qgl-query.scw PDB file : Tito_Scwrl_4QGL.pdb: