Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSPQGIEVPDALVASYTRNGGEEERAWIARLPALVAELLDRWKLERDGGIRSGEASLVVPVRRTDGTRAALKLQMPR-EETTAALIGLRAWNGDG---IVRLLDHDP--------ESSAMLLERLDGSRTLASVEDDDVAMTTLAGLMARLHSVPAPEGLR--GLGDLARDMLASVPDALA---AL-----PNPED-------RRRLHGWASAVT---------ELVG-EPGDRMLHWDLHYDNVLAAEREPWLVIDPE-PLIGDPGFDLWPALDTGWERIHSTGNAPRVVRRRFDLLTEALGLDRRRAAGWTLARLLQNTLWDIEDGRTDIDPSQTIVAEALPRH 4PDY Chain:A ((17-290)) ----------------------------RSQAPLIPQAVVSKYDLAIQ-QR--HADGNIEVWTDSKGRRYAAKRSSIAPAHCRIMVQCLRHAQEQGFTKFARFVTTSSNAPYVRHGDFTYYVTEWVSGQ-PANFG--LPEHVAQTAYTLAQFHEATRSFRTDWK--DDVFGLFQARWRDLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERG---NWDGHLAYACFLHFDAVRNIPKS---------------------------------------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 856 -8565 -10.01 -37.40 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -10.01 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.260

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: