Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMCILALAEKNQNPEKPSF-LEDTFQEIEAPAAAMLSYGGHEIHFELDKNCWPRLRHGSAVPSGYSAHVY------------------------------------------------------------------------------ 3H9V Chain:A ((7-336)) GTLNRFTQALVIAYVIGYVFVYNKGYQDTDTVLSSVTTKVKGIALTKTSELGERIWDVADYIIPPQEDGSFFVLTNMIITTNQTQSKCAENPTPASTCTSHRDCKGFNDARGDGVRTGRCVSYSASVKTCEVLSWCPLEKIVDPPNPPLLADAERFTVLIKNNIRYPKFNFNKRNILPNINSSYLTHCVF---SRKTDPDCPIFRLGDIVGEAEEDFQIMAVRGG-VMGVQIRWDCDLDMPQSWCVPRYTFRRLDNKDPDNNVAPGYNFRFAKYYKNSDGTETRTLIKGYGIRFDVMVFGQAGKFNIIPTLLNIGAGLALLGLVNVICDWIVL

General information: TITO was launched using: RESULT: Template: 3H9V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 76 4655 61.25 71.62 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : 61.25 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_3H9V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3h9v-query.scw PDB file : Tito_Scwrl_3H9V.pdb: