Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLY---------PLTNYTFGTKEPLYEKDSSVAARFQRMREEFDK-IGMRRTVEGVLIVHEH--RLPHVLLLQLGTTFFKLPGGELNPGE-DEVEGLKRLMTEILGRQDGV--LQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN 1QWT Chain:A ((17-255)) ENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSL-TDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVFDLGPFIVDLITFTEGSGRSPRYALWFCVGESW-PQDQPWTKRLVMVKVVP--TCLRALVEMARVGGASSLENTVDLHISNSHPLSLTSDQYKAYLQDLVEGMDFQGPGES

General information: TITO was launched using: RESULT: Template: 1QWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1144 -5289 -4.62 -23.72 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -4.62 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.225

(partial model without unconserved sides chains): PDB file : Tito_1QWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1qwt-query.scw PDB file : Tito_Scwrl_1QWT.pdb: