Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAVRVLVASRLAAASAFTSLSPGGRTPSQRAALHLSVPRPAARVALVLSGCGVYDGTEIHEASAILVHLSRGGAEVQIFAPDVPQMHVIDHTKGQPSEGESRNVLTESARIARGKITDLANLSAANHDAAIFPGGFGAAKNLSTFAVDGKDCKVNKEVERVLKEFHQAGKPIGLCCIAPVLA--AKVLRGVEVTVGHEQEEGGKWPYAGTAEAIKALGAKHCVKEVVEAHVDQKNKVVTTPAFMCETALHYIHDGIGAMVRKVLELTGK 6F2F Chain:C ((1-166)) ------------------------------------------MKVLFLTA-----NEFEDVELIYPYHRLKEEGHEVYIASFERGT---ITGKHGYSVK----------------VDLTFDKVNPEEFDALVLPGGRAPER-V----------RLNEKAVSIARKMFSEGKPVASICHGPQILISAGVLRGRKGTSYPGIK-----------DDMINAGVEWVDA---EVVV--DGNWVSSRVPA----------DLYAWMREFVKLLK-

General information: TITO was launched using: RESULT: Template: 6F2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1011 -61191 -60.52 -373.11 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -60.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_6F2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6f2f-query.scw PDB file : Tito_Scwrl_6F2F.pdb: