Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSYAHLNHYISPSAEAGLSDAWRRSLGRLGDYSAILIRSLNLRPLEVSLHDQTEEHILVRIRLP-GSYLILRVAPED----DLAAYVYQIRTLNGHN--IPAPQIIQRDLSRSLVPFAFTLESYVPGVTAIELQS-----------APLLRGAGRQAGRALRRMHRIPVTSAGRPTASGRWTQQNWRTVLRQIGRRLAAPPADALIFGEREQRVVADLLDHPV--LECQQPSLIHGRFSPRAVRCTSSGNVHVEALIDPGHYVGGDGLYDLACGLCAAYPEPWREGLLDGYQSAGPLN-EAERRRLPLLQLLTNYWEACRLYMHAEPHEAARSEAIRLMEEQVGLTRLVSV 5UXA Chain:A ((22-298)) ----------------------------------------ETIQFNESGL-DF----QAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT-------EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLV-VHTPEEARMSMKQRMDAVRAK--FGV----GENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDA--NVTGLIDWTEAKVTDVSHDFIFNYRAFGEE-GLEALILAYKEIGGYYWPKMKEHIIELNAAYPVSIAEFALVSGIEEYEQM--AKEALE-----------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1104 -638 -0.58 -2.49 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -0.58 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: