Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLTDPPSLGTAQAASVIAAAVRLLTKRTGAEVTLVEVEDLGGTGQTLVVRARVGENPFSLPRTLVIKQTPPQGSREMFTREVVSYQFTTSLATGDRPGPELLAWDSEERVLIISDLGEATPMSQVLASQSGGEENALMAWAQRLGRMHAATAGREIDFAALLRRAKVPPSRDIIGQQVQGAVLALPRQLREQLSLETPEEVRAIAAKAKELFDKGTMRAFSPADPGPDNILLTADQGVRFLDYEWGGFRDATLDIAYALVTYRGAMRKEESKEYWSELDQRIVQAWRAEAVAVWPALADDVTLQKRVLGAMLIWVWLTTAWWLPGHGRDGAWSHRHSLSTPTPQALMARWEGLLGLANAAGDLAVAEHAETVIAALRAAWRW 2Q4L Chain:A ((23-351)) PELRKDPVTNRWVIFSPFKSKSCPFCIGREQ-------------ECAPELFRVPDHDPNW---KLRVIENLYPALSRNLETRTIVGFGFHDVVIESPVHSIQLSDID------------------------PVGIGDILIAYKKRINQIAQHD---SINYIQVFKNQG--ASAGASMSHSHSQMMALPV---------VPPTVSSRLDGTKDYFEETGKCCLCEAKSKH----FVIDESSHFVSVAPFA---ATYPFEIWIIPKDHSSH-------FHHLD-------DVKAVDLGGLLKLMLQKIAKQLNDPPYNYMIHTS---PLKVTESQLPYTHWFLQIVPQ-----LSGVGGFEIGTGCYINPVFPEDVAKVMREVSLT

General information: TITO was launched using: RESULT: Template: 2Q4L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1615 -120811 -74.81 -401.36 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -74.81 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.208

(partial model without unconserved sides chains): PDB file : Tito_2Q4L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q4l-query.scw PDB file : Tito_Scwrl_2Q4L.pdb: