Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNWSFGIDNDKLIGLVLEGKKTATSSLYNFDKIPVIGKESIIHFDNEKDACIVETVDYKIIKYNEMTEELAKLEGEGDLSLNYWKQVHLNFFKSVNPNFKEDDKIIFEIFKVTKNLVEERLKLGKSIASKNTDLLGNIVKVEEINSGFNNTLFNINDKYVIKVCTNKELENTFENEKD---FYLSNKDNCFIPKLYKYDDSKVDCDYIYEVIEKIKGKTLYYYWYKMNEADREKTIEKLIDIIKKFHSVNGKEYDWKNKIKGEIKNRIIKCKKYFKIKDYNMILESIDKYDEYLSDNKFALIHNDLHFDNVIYNNGKLTIIDFNDSIQAPIDFEFRLLYMCQEQPWKWADI----------EMDPYQKPEDYKNIWNYIKKYYKELNEIKFLEQRMIIYRIWNDTGHLKKYHNVELTKGIVENSKKLI 3GLG Chain:E ((1-334)) MRWYPWLRPDFEKLVASYQAGRGHHALLIQA--LPGMG----------DDALIYALSRYLLCQ-----------QPQGHKSCG-----HCRGCQLMQAGTHPD------YYTLAPE--KGKNTLGVDAVREVTEKLNEHARLG------GAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATRE-------------------PERLLATLRSRCRLHYL-------APPPEQYAVTWLSREVTMSQDALLAALRLSA---GSPGAALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALN----------------HEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQ-------PGVVLPVPHL-

General information: TITO was launched using: RESULT: Template: 3GLG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1486 -32439 -21.83 -101.05 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain E : 0.66 3D Compatibility (PKB) : -21.83 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.200

(partial model without unconserved sides chains): PDB file : Tito_3GLG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3glg-query.scw PDB file : Tito_Scwrl_3GLG.pdb: