Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence AQKI---CHFTILKMYK---NTWTFVRKDSAPADQTTTIEGIKVFFSKDCIPQHDY 1H8A Chain:C ((79-126)) KTSWTEEEDRIIYQAHKRLGNRWAEIAKLL-PG---RTDNAVKNHWNSTMRR----

General information: TITO was launched using: RESULT: Template: 1H8A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 123 12712 103.35 302.67 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : 103.35 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_1H8A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1h8a-query.scw PDB file : Tito_Scwrl_1H8A.pdb: