Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------RFCVYYDGHLPATRVLLMYVRIGTTATITARGHEFEVEAKDQNCKVILTNGKQA--------- 2MYW Chain:A ((7-85)) SGRENLYFQGHMAAPARFCVYYDGHLPATRVLLMYVRIGTTATITARGHEFEVEAKDQNCKVILTNGKQAPDWLAAEPY

General information: TITO was launched using: RESULT: Template: 2MYW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 101 4944 48.95 91.56 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 48.95 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.658

(partial model without unconserved sides chains): PDB file : Tito_2MYW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2myw-query.scw PDB file : Tito_Scwrl_2MYW.pdb: