Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KRCDVGIYNSDGTGPRAYKNIDIPASCSQWDFDFEYGGVTYTVRLHYEGCRTEIIGRPQLPSHLIIRG---------------------------------------------------------------------------------------------- 5IVA Chain:B ((3-154)) NTSGFQL-RGLGDAQFALKEIDVSAR-------NAYGPTVRELKETLENSGVKVT--SNAPYHLVLVREDNQQRTVSYTGSARGAEFELTNTINYEIVGANDLVLMSNQVQVQKVYVHDENNLIGSDQEAAQLRSEMRRDLIQQLSMRLQALTPAQLDEAQR

General information: TITO was launched using: RESULT: Template: 5IVA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 122 -12636 -103.57 -217.86 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -103.57 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.113

(partial model without unconserved sides chains): PDB file : Tito_5IVA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iva-query.scw PDB file : Tito_Scwrl_5IVA.pdb: