Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDISIIAAQLVKEKVISHNPNSVKALNGGTTSTVYLLDEKYVVKLNEA----EVIREEAHFLSFYEG-NTLF-SKLLYKEP---LHTYIVYSFLEGSTWCEQGH------KRSTLRTLVKEVINKYKIVPEADGWGWKENPVQSWNEFLTTNVMEAYENVRRYISEEEYRTVLKLAN----R-DAGVNQPFLLHGDCGFHNFIFRE----NRLHGVIDPL-PVLGEPLYDLIYAFCSTPEDLTKETIAYAMKQCLFHKKERDLYEEIVIGLYLRIDTCLRHHPKDLEEYLVAWRYWMDEINPAL 3TDW Chain:A ((4-253)) --NKLHYTTMIMTQFPDISIQSVESLGEGFRNYAILVNGDWVFRFPKSQQGADELNKEIQLLPLLVGCVKVNIPQYVYIGKRSDGNPFVGYRKVQGQILGEDGMAVLPDDAKDRLALQLAEFMNELSAFPVETA-ISAGVPVTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYMTHPVYTRYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDCGELF-TRQVMAYRGEV--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 891 -38971 -43.74 -173.20 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -43.74 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: