Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MSVEVNSEETVPEVLLLDLGGITFPSPIGPLLAAAESIRDPGLPRRMVSSVLWKELECGFLTIPEFCEQFAKGKAVVQEALIGWFEGLRAMEPSPRMIILLSELRLKYPGLKIGAVTNNFKHPAPLRPMRDLRGELL-FDTIVESAVEGMAKGHPGGERWALVEVWNFHDGSYMEAHIIWSSVR------------------LFKTALERLDYHGPASAVLYLDDLKHN-LAQPASLGIRTRHAITP---EEATETIRKMLALDVSSGSPLQKYLASLGVLPDSISDQARPFAWQFSFGQSNPTYLVGLIPEK-PLEGYTLQKLLEEKCAVLRKQP----------------PGELLPSAHDVVREY-------TVMSELGKEGCVPVPRTLG-----LCTDKTICGAAFYVMRFVTGVVFTDPNLPNLQPYQRR--------KVYEDLMKTAAAVHNF-PWHRSEALTQRFRGDPSKYVSKQVERWSTQGIDVEGFKRLA------------KGLLDNVKISQKSPEFQSLVHGDFRLDNCIYDPHTFEVV--------AVLDWELSTTGNSLVDIA--SALSQYYGLY-PGIAQLSSNFEELGIPKERELLDLYLRYREVESIPEGLMQYMEAFQCLRMAG---------ILYGVLMRTKKGNASQQH--------QQAKGGSIFSMAYVTKL-----CTRGLELLQSRACSSSPIAVDAPSKL---------- 4KVM Chain:A ((5-731)) QLSPKEITLFRTALKCYETKQYKKGLK-AIEPLLER----HPEHGESLAIKGILLHSLG-----------NTKEGYDNVRLGLRNDVGSGVCWHIF-GLISRADKDYVQAAKCYINAHKLEKNNSSLLRDLALLQSQLRQYKALADTRNALLQDNPGVRANWSALAVAQFLRGEYASAYKIVDAFESTINQGVPVDTQEESEAMLFMNLVILKK-DGVEDAYKHLLSIEKKVLDRVAFLETRAEYELYLSKMEEAKSTIYLLLDRNPDNHQYYYNLQRAYGYEDAS-GKVLDSAEWLNLYSQLAKRYPKSECPTRLPLEKLEGDEFLTHVDLYLRKKLKRGIPSVFVDVKSLYKDTKKCKVVEDLVSKYASSLSTTNKFSEDDDNSQIEIPTTLLWTYYFLAQHFDHVGELEKAEKYVDLAIDHTPTLVELFMTKARISKHKGELQTAMEIMDHARKLDLQDRFINGKCAKYMLRNDENELAAKTVSLFTRNEAVGGAVGDLADMQCLWYMLEDGKSFARQKKFALALKRFSTVFKIFDTWADDQFDFHFFAFRKGSLRTYLDLMSWEDSVYDDPSFREAAQGSIEIYFALFDLPFAKYSPKLPDFEKLSSGEI-NEEEEKKIYKKLKKDLSKRLERAEKLKEADKSRKYDEDPLGENLVATSEPLKEAQKCLEKLLPYGDKNPSAYILAAQLYTRLKNFDTASKYLEQAKVILGQNDPTVISTEKFYNSIKTQSNAA

General information: TITO was launched using: RESULT: Template: 4KVM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2534 80871 31.91 134.79 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 31.91 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.187

(partial model without unconserved sides chains): PDB file : Tito_4KVM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4kvm-query.scw PDB file : Tito_Scwrl_4KVM.pdb: