Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MLAERGRLLLTVVGELLAAEVCAAGTRIRGPDERARRERQRALRIRTALERL--------GPLYV----KAGQVLSTREDLVPPAVARELESLHDRAAPCPFDR------MSAVLEAELGPD--------WRR--------RFRHFDTERPLGCASI-AQAYAAVLDDGRSVVVKVQRP-GIAAQMTTDMRLLRGM-----TRTLARRTPVLSETIDFEAMLAVIFEA----VRNELDLTLEAANMDEARAAVTAVPGID--VPGVVHATERVLIQRRAPGTSVRDA-DPA-AFDDAERERVGTGLLTVMYQGYFIDHRFHADPHPGNVFVCPGGPTTLIDWGMI--GRLDRHLSSTLLMTLLGLANNDAHAVAR---A-WSEMGRPTPWADI-GAFEQDMSV-------VVPRLASIPLDRLRFGASLATLLRLSTRRGIRTNPMIGLLGKSFANMEGTVRHLAPHLSATDV----LVRQTG--RVLGEYAAQSLSQQQLG-FTALQLLSGLETLLPHA-----RAVGRSLSGGDL-T-LQHTAVTRPFSLVDHRSSARIRRAEHHLLGAAALVWLLRRRGRG 3LMM Chain:B ((9-574)) EGRREQLIAQVESILASAADGRVQKTKETQSVDFKEEAGRRNGPQIEPGKPENPEAADKLADEVACMANTPGGGALIVGIEDKTGRIIGT--ELDIDWLRQGIFTRIDVAPDVVAKRVLGQRVLAIYVAAAAEPIEDTSDRLRWRVGDSCRPVDRAEWWEYQRAQSGFDPMAQVTTATLGDARPAALALARKWDPAFAELTDEELLRGIGALDAEGFLSQAGKLLFTSLDRTAIELSIFDVHGGQVLNRVVPEPEKSCLEQLDYLEQALNVVNKNVPEI-------------PRLAVREAMLNAMIHRDWNRSEP-------------IDVRWI-ELDSTLIVRSPGGFPAAITSENVLSNRAARYPALADLYRALGLVDKQGVGVDRMYQAMIALGHRPPTIEEIAGPFVETTLVGGRPVLPVLELVSSIVPEARQDDYRIAIVLYLLFQRPFIT---IDVVARGLQSGKEAARNALEAARQTTVAGAPLIIAHDGVWLLGNACREILRKVEPSPFSPVRYLSTDQAELTNAAMLWLSEVG-DLATSDLMAMCGVSRGTAKACVDGLVDEERVVAVG----GGRSRRYRLV-----

General information: TITO was launched using: RESULT: Template: 3LMM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2448 59841 24.44 128.69 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 24.44 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.116

(partial model without unconserved sides chains): PDB file : Tito_3LMM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3lmm-query.scw PDB file : Tito_Scwrl_3LMM.pdb: