Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQTLPPDSSASWGAWGASASTSLEAYRALRADRARWLPVALDIARGHGLDISSPHVFSTGTNLVVGLGERLILKIFPPQLRAQFVSESGSLTQLAGRLSLPIPEIVAEGARDGWPYLVITRLTGTLGSEVWPSLP-----------EDQKERVLRQIGETIASV---------QRAPLGPLASIEPRWDAFMRQQMQGCRARHERLGLAPKFLVGLD--DLLREAAAG--LIPMDAPPV----ILTGEYIPEN---FLLACDDGEWSLKGLFD--FGDVRAGFRDYDLLGAS-------AFMAAGRPGRVRNLLEGFGYAKPDFTLKRRLMALMLLHQASDLNSHICIAGWQEKANDLVELQELIWAS 4FHL Chain:A ((2-392)) --------GSMTLSSNQYQLPLNVRPYTTTWCSQSPSCSNLLAIGHDTGITIYCASEESTG----LTLQELFTIQTGLPTLHLSF-SSSCSYSENSPVYSLFLACVC----QDNTVRLIITKNETIITQHVLGGKSGHHNFVNDIDIADVYSADNRLAEQVIASVGDDCTLIIWRLTDEGPILAGYPLSSPGISVQFRPSNPNQLIVGERNGNIRIFDWTLNLTELVKNPWLLTLNTLPLVNSSLANVRWIGSDGSGILAMCKSGAWLRWNLFKNLLPNVQGISLFPSLLGACPHPRYMDYFATAHSQHGLIQLINTYEKDSNSIPIQLG-MPIVDFCWHQD-GSHLAIAT-----EGSVLLTRLMGFT

General information: TITO was launched using: RESULT: Template: 4FHL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1672 -70619 -42.24 -231.54 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -42.24 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.070

(partial model without unconserved sides chains): PDB file : Tito_4FHL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4fhl-query.scw PDB file : Tito_Scwrl_4FHL.pdb: