Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHPTPSPDTILFASHTTATAASVAQWVERHYDLP--VQQCYLIRRN-LNDNYAVRAADGSRYVARLCAIRPR-GPFNV-DFEVALLAHLEAQGVGVASPVLAANGATSVALPFPEGPRVLVLFRHCEGSVPD----TPEDLHLTGATLAQIHSAAQSYTGPASRY-VLD---GHHLAGRTLDYLAAH-PE---LAPSVLDAYRELIHRLLGELANV-EDTLTRVMCHGDTHGFNNHVYS--DAEGARRAAFFDFDDAGPGFLAYDLCVWLWSNLPRKAPM----EPDEALLQRWQQYLAGYRA--GGGHVTDADLAALPLFVQLRHLWNMGEGVARIHHWGANMMSVDWIKKQLEVMAAWARLDLPA 4OCV Chain:A ((24-326)) ------------------TNEALFD-VASHFALEGTVDSIEPYGDGHINTTYLVT-TDGPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEI----DGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFDANQLTETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGK----ARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELR-DSITAREAELLPFSGNLLTMECGMR-----------------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1209 -10730 -8.88 -38.74 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -8.88 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: