Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTRRPEIPADLRQWIAEMTGTDVVEVRQVPGGASRQAW----FVDAGTGARSR-ALFLRYDPREADPGSAFHPLQV------EAEIMAELHRHGVTVPQVIAAHPTLQAVLLERVGGDTWFRLIQDPDE------QVRTARD---FI----GKLAALHRIDAQHLTIPSLGPAGP----VAGHVRAEIAAMRARLSRYGKPAPLLAFCIDWLDRHVPD------YDGPTVMVQG------------------DTGPGNFMYSGGVVTAVVD----WELAH-FGDPMDDIAWLSLRTVQDTFTDFPARLAEYEQLSGHRIDDDRVWYYRLFAETRLASISPGSIDTRASVPPASPDAGNSLIYGMLHRRLLVEALAHVVGIPEVDVELPPEGDADAPSEHSSVYEAAASAMSGAAARSTDALALRY-VKGAARLVKYLAEVDRIGAVVDAQETAELTAVLGWAPRSVSQ-GRAALAELAGRGDLTDRDYV--TQLWRGIKHDDYLTRTASGALARRTWPPLRHPVPPPSVEQGDRDRWVSPSPKTTVS 5KDS Chain:A ((20-530)) HMASVLEL---------EMRGDSISEAKK------RKVWNFQDWQITGLSARAGDKITVYVDVAEGDPTPTLLYKQSLTQHGGATSFQLKPGKNEITIPEINYESNGIPKDVIQ--GGDLFFTNYKSDSQKRAPKVRIEGASKYPVFILGKSDENEVMKELEAYVEKIKAEPKTTPNIFAVSSNKSLEFVQATYALDWYKKNNKTPKYTAEQWDQYIADAMGFWGFDNSKDVNSDFNFRIMPMVKNLSGGAFMNAGNGVIGIRPGNQDAILAANKGWGVAHELGHNFD----TGGRTIVEVTNNMMPLFFESKYKTKTRITDQNIWENNTYPKVGLDDYSNNELYNKA-------DSTHLAQLAPLWQLYLYDN--TFYGKFERQFRERDFGNKNREDIYKSWVVAASDAMELDLTEFFARHGIRVDDKVKEDLAKYPKPDKKIYYLNDLAMNYKGDGFTENAKVSVSTSGSNGNIKLSFSVDDENKDNILGYEIRRDGKYVGFTSNDSFVDTKSNLDEDGVYVVTPYD-------RKLNTLNPIEVN

General information: TITO was launched using: RESULT: Template: 5KDS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2377 92147 38.77 204.77 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 38.77 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.086

(partial model without unconserved sides chains): PDB file : Tito_5KDS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5kds-query.scw PDB file : Tito_Scwrl_5KDS.pdb: