Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVLASNVFVSDPF--NSANDPKMPFLAEALNP--IKVQKYLT-----KALFNQFKNIQLHAIRVIRH-----------KLGRRCAIAYELTVDTQSLVIIGKARA-------KGLDHHSYELQRSLWQA---------GFSEDSSDGISVPEPLGIIPEWQMWLQKKVP------GTIATDLLP--GTEGVAIASKIAEAAHKLHQTGIFTRRC-HTMSDELNILHERLPQVAQQYPQ------WEKR----LEQILSKCDRLGANTPELSQC-GIHRDFYGDQV--IVNGEHLYLIDLDLYCQGNPA-----LDI------GNFIAHITEYSLRILGNPNALADREAAMAERFIQFHGEAIRLGIQSYTTLTLVRHIYISTQISDRSSFTEAILELCEQRLR 4UUW Chain:A ((1-394)) MERAEILGVGTELLYGETLDTNTAEIARSLKPYALKVERTLRVADEVAPLAREVEEAFARARLVVLSGGLGPTPDDVTREAVALALGEPLELDEAVLGEIEAFFRARGRAMPEANRKQAMRIPSATWLKNPRGTAPGWWVRKGGKDLVLLP---GPPPEWRPMWQEVLPRLGLPRRPYAERVLKTWGIGESEIVERLGPLFVREEEVEVGTYPKVHGVEVVVRGREDRVAELAERIKKKLLKEVWGEGEMTLAEAVKRRMEREGAT---LSTMESLTGGLLGAEITRVPGASRFYLGGVVSYSVGAKARFGVPQDLLSRTVSAETARAMAEAARSLFGSTYALATTGVAGPDPLEGEPPGTVYVALAGPTGAEVRRYRFPGDRETVRLRSVYAALALLVT---

General information: TITO was launched using: RESULT: Template: 4UUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1486 2344 1.58 7.21 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 1.58 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_4UUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4uuw-query.scw PDB file : Tito_Scwrl_4UUW.pdb: