Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVRIPDELNAQRRLGPDWENWLDRLPRLCDEIVSDWGLTVVGEPTNGFASIVIPVSTSD--------G-DAMLKVGFDSSPETEHEHLALTYWAGDGA-VTMFRADPARHAMLLEQLHSRDLSQEW--DLQACEIVAGFYSRLHRPAPPR---LRPLTGFVDRWLGALEADARE----M-PV---PRRLIDHALNRGRAFVDDPASVGRIIHGDLHYENVLAADR------EPWLVIDPQPM-SGDPHYELAPMLWNRWDEMD-------------GYLRESIQRRFYTLVEAAGL----------------DDDRARDWVIVRMILNAHWAVEDAKRMNRALDADERDWITRCIAVAKAVQR 5FTG Chain:A ((39-351)) ---------------------------------------------GGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---EAMVLESVMFAILAERSLGPKLYGIFPQ--GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKI-------------------------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 771 10607 13.76 46.32 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 13.76 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.259

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: