Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------MNNWSFGID-NDKLIGLVLEGKKTATSSLYNFDKIPVIGKESIIHF--DNEKDACIVETVDYKIIKYNEMTEELAKLEGEGDLSLNYWKQVHLN---------------FFKSVNPNFKEDDKIIFEIF----KVTKNLVE--ERLKLGKSIASKNTDLLGNIVK----------------------VEEINSGFNNTL------FNINDKYVIKVCTNKELENTFENEKDFYLSNKDNCFIPKLYKYDDSKVDCDYIYEVIEKIKGKTL-YYYWYKMNEADREKTIEKLID-----IIKKFHSVNGKEYDWKNKIKGEIKNRIIKCKKYFKIKDYNMILESIDKYDEYLSDNK----------FALIHNDLHFDNVIYNNGKLT------IIDFNDSIQAPIDFEFRLLYMCQEQPWKWADIEMDPYQKPEDYKN-IWNYIKKYYKELNEIKFLEQRMIIYRIWNDTGHLKKYHNVELTKGIVENSKKLI---- 4NFU Chain:B ((1-540)) SQDPESSSSLKGSALGKLVVTSGLLHSSWSKILEIHNPDSGLEFQIHREEKFTLVVFSAPPICRSSSSDSTLLHVKDKENPFPFLCSENNPSFSLHTPAFNLFTSA--STSLTYLKSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIEPTLKRPLCITFGSPLIGDASLQQILENSVRNSCFLHVVSAQTRIKMDFFKPFGTFLICFDSGCVCIEDHVAVTELLNGVHDSGLVDYSQVLNRLDQSMLSLADSRLIPEDVIKGIEKRAEMKNLRFDMMFKKLNDMKISMAYIEWYKKKCKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRP----QSDASILKRRFLFSGNNYRRMIEPLDIAEYYLEGRKEYRTTGRSHHYVMLEKWFGMESILIEKERCKKRDLSDLLTFDSCFWAEVEDSLIVINQLNTTVGMRDDVREVLTRKLVEFEGYVWEIITK--REVSPEIFLEES-SFMKWWKEYKKIKGFNSSYLTEFMNTRKYESYGKSQ

General information: TITO was launched using: RESULT: Template: 4NFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1964 89675 45.66 220.33 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 45.66 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.185

(partial model without unconserved sides chains): PDB file : Tito_4NFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4nfu-query.scw PDB file : Tito_Scwrl_4NFU.pdb: