Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSSAPYNEQVIRDITKILNANFSFPTSITNIELLVKGM-NYTLRVECK--NTKYYLKIFSASRDKI-DISFEMHVINRLSASGIGVATAINSINGDAYINLTLAGEDRLAVLYAHVGGRTLT----SDPRDIFLFSKALAEIHRTPTSFFLQ-YT----------S--RIFSIESECYRIRSSIEKH-----------SYIPKPLASKLLDNCYSIQALASTDC-AVLSHGDAWLGNAKYV-N-GVIYFIDLEDSKLANRNFDLGVMAYNLIVKK-----YEVRENIQALLRGYNELNTSIT---SGSIKPY-IQLRSLFVLCFLLENKL---VDQSLLQKVFERAEYFTSSKFDSEMAHLP 2QG7 Chain:E ((110-454)) ----------------------------SLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNKNIA-KKIYVFFT-----------N---GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLC---KRENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSS-DFDFINYGMTRLTASCLPIF--------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1028 -36854 -35.85 -141.75 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain E : 0.60 3D Compatibility (PKB) : -35.85 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.270

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: