Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLEPWLHRYWNIGPARMQALASGHTNKTYLVECDAGRAVLRVSWSG-KPVGQVDREAAILGHLGETRAAPMLPALPRLRPTVDAQSGVRLP---DGSWLHLFEHIDGSP-GL--PPEAETGAIDAMRALAHLHAALAAIPVTES--A---PLAWLSARHARVAARAA-----PPLPGNLSDRYGTMIRRIGAHLDAAARWLTGTVHWLHGDYHAGNLLYVGG---AVNGVLDFDDVGQGAQWLEAAFALFALSRDAGRDDRFVFDARRWDAGLQAYAATRP------DAVPDWMHDHRDALMALFCADQTLIHLEAAQRGLWVPGPGIGFLGGWRQLLDGAAP 6EF6 Chain:A ((31-313)) ---AQQALTHYDVSDNASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTD---SDVT-VPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTL--GRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRK----LEEY-GSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHD-------PRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLL-------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1114 -25101 -22.53 -98.82 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -22.53 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: