Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQLRAELSHLLGEKL-SRIECVNEKADTALWALYDSQGNPMPLMARSFSTP---GKARQLAWKTTMLARSGTVRMPTIYGVMTHEE--HPGPDVLLLERMRGVSVEAPARTPERWEQLKDQIVEALLAWHRQDSRGCVGAV--DNTQENFWPSWYRQHVEVLWTTLNQFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMVGPGLMLWAPREYELFRLMD---NSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVNTGRFSRRNFDLASKSLLPWLA 5IGI Chain:A ((17-257)) ---------ARHGLKLHGPLTVNELGLDYRIVIATVDDGR--RWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWV-VPQDSEVFAESFATALAALHAVPISAA-VDAGMLIRTPTQARQKVADDVDRVRRE--------FV-VNDKRLHRWQRWLDDDS-SWPDFSVVVHGDLYVGHVLIDN-TERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGR-------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 939 -1248 -1.33 -5.45 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -1.33 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.209

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: