Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------PWEWVT---WKDFCRLTLKKGDEIQSQSKWVAKPGPIGILIQPGVYESFKTDKDCNPKIRRGKLPDG--YTLHGQVF------------------ 5J4F Chain:A ((22-119)) EVVHFLEGVCFEKLHIEVLNENSSHKEIRICMPKGAVMDKHKA----PGAISVQVLEGKIVFEVGD-------EKIEMPKGALISLEAQVLHRLDALENSVIRLSLSKK

General information: TITO was launched using: RESULT: Template: 5J4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 138 4508 32.66 73.89 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 32.66 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_5J4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5j4f-query.scw PDB file : Tito_Scwrl_5J4F.pdb: