Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------SVRNPKYGDH---CELTVWIHYKGKDIP-LMRYYVEIG--GDLTFDEKDGAWKHCV----------------IKTTKQACE----------------------ATW------TGGSKSEPCPPKNINVD------------ 1M5X Chain:A ((6-166)) TLQPTEAAYIAGFLDGDGSIYAKLIPRPDYKDIKYQVSLAISFIQRKDKFPYLQDIYDQLGKRGNLRKDRGDGIADYTIIGSTHLSIILPDLVPYLRIKKKQANRILHIINLYPQAQKNPSKFLDLVKIVDDVQNLNKRADE---LKSTNYDRLLEEFLKAGKI

General information: TITO was launched using: RESULT: Template: 1M5X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 196 20052 102.31 263.84 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 102.31 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.303

(partial model without unconserved sides chains): PDB file : Tito_1M5X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1m5x-query.scw PDB file : Tito_Scwrl_1M5X.pdb: