Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------------------------------------------------------------------------------------------------------------------------------EQARYHGQCVVEIYNSQNQRADILHSQIWMRETLPDYTLHPLSRYAVLV--DGDCNARAEYGIAPPGNTFRGCTAYE---------------------------------------------------------------------------------------- 5FQN Chain:A ((73-401)) LYYECDVSVHEEMIADQVRTEAYRLGILKNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRLNGLEDRVHVLPGPVETVELPERVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFVAPISDQMLEWRLGFWSQVKQHYGVDMSCMESFATRCLMGHSEIVVQDLSGEDVLARPQRFAQLELARAGLEQELEAGV---GGRFR-CSCYGSAPLHGFAVWFQVTFPGPLVLSTSPLHPATHWKQALLYLNEPVPVEQDTDISGEITLLPSPDNPRRLRILLRYKVGDHEEKTKDFAME

General information: TITO was launched using: RESULT: Template: 5FQN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 206 -7101 -34.47 -100.01 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -34.47 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.113

(partial model without unconserved sides chains): PDB file : Tito_5FQN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fqn-query.scw PDB file : Tito_Scwrl_5FQN.pdb: