Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHGRSRLYRYLKSGRLSAKAGFDRYPDLRVKQL--GGSHNVYLVSDHRSHKKCIVKSFAGSGPKSVQHMEKEYKRLRQAGRLVDDRRWVHVARPLCKSNGEGFFAEELVSGKALGDYMRDAMANGRDSELYEKLTMLAGFLALLHKKTERPSHVKPS----------NIRDELK---------KHARQASKGGAFAPHELKRIESLVDNVTSNDLISQTRRGLVHGDANPSNFLYDKKRLNVIDMERSGYRDPVYDLGMLAGELCHYAMKYGGNEYKADPFIGHLYWTYAGNFKDQLGTFIRLTGRNPIYMANSLLRIARHPFFSPEYKRRLARKAYECLKSLKK 3F7W Chain:A ((12-286)) --------------------LTGREV-AAVAERGHSHRWHLYRVELAD-GTPLFVKALPDDAPALDGLFRAEALGLDWLGRSF----GSPVPQVAGWDD--RTLAMEWVDERPPTP---------------EAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDAL-DHLAGDPEPPARIHGDLWNGNVLWQDDGAVVIDPA-AHGGHREADLAMLALFG--------------LPYLDRVRDAYNEVAPLAEG----WRARIPLHQLHPLLVHVCL--FGAAYRTTLVDTARAAL-----

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1317 22773 17.29 89.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 17.29 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.340

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: