Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------MTILTKLTQKDFEEI-----LLRYSIGKYKAHEHISYALQNTVFILNTTKGKYVLKIFEKSE---------------ADFVKFQIRITDYLYHKKVPVARIVKLKNG-RNLLMYRNKL---------MAIQKFVGGNPAKKLNKELAGNLAKNLALMSKHLLKLKLKGIFMWSNDHEFET---------NTDIENIEKVNFVGYEKKLLEEIGKLDR---------NNLRRSVIH--GDFHEMNLLTENNELRAIIDWDDAHEDYISYE-----IAVYLVSYFDAVSIKRSKEYIELFFKEFQRYIKLN-EEEIKAVYYFMKQRLIGVIFWHQNQK----KIHKNFEKRLDRSMKKLITRYVALENITLDEFMQLAERKSTSQKHHRAELKNKKNLYSVIVQD 4D10 Chain:C ((3-403)) SALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLAVLFVKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGILKQAIDKMQMNTNQLTSIHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIED--GEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQ-EMLKCIELDERLKAMDQEITVNPQF-----------------

General information: TITO was launched using: RESULT: Template: 4D10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1364 14584 10.69 45.15 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : 10.69 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_4D10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4d10-query.scw PDB file : Tito_Scwrl_4D10.pdb: