Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVYTVLDSGAFARIAG-AFGLGEVQTVDAIPEGSINTNHR--VLTSSGRFFVRHTTVRSAEVLRFEAALLAHLAESHFP-GPTPVLTVQGEPFLELQGGRVTVFRWLAGEELQRSQLTPVHLERLGHELGKMHRLTQSFGGSRDN--PYSAAQVQVWLKGLRSNSD------A-------EVASVAVELEGYLARAE---QERGGGLEPQGVIHADLFMDNVKWLADRVGAFFDFEMACRDAYALDVAITLNAWCFDGDYLPELCQAFFRGYQDARPLSAVEREHLFGHALFGAVRYTASRIRDFHLSPLPAEQLTRKSFRTYLARARTLVSLGPAGFLARMGL 3I1A Chain:B ((4-286)) ----QPIQAQQLIELLKVHYGI-DIHTAQFIQGGADTNAFAYQADSESKSYFIKLKYGY-H--DEINLSIIRLLHDSGIKEIIFPIHTLEAKLFQQLKHFKIIAYPFIHAPNGFTQNLTGKQWKQLGKVLRQIHETSVPISIQQQLRKEIYSPKWREIVRSFYNQIEFDNSDDKLTAAFKSFFNQNSAAIHRLVDTSEKLSKKIQPDLDKYVLCHSDIHAGNVLVGNEESIYIIDWDEPMLAPKERDLMFIGGGVGNVW-NKPHEIQYFYEGYGEINV----DKTILSYYRHERIV-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3I1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1025 -19383 -18.91 -74.26 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -18.91 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_3I1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3i1a-query.scw PDB file : Tito_Scwrl_3I1A.pdb: