Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNKMKNHKITKLGGLNNSNYLLECEN---------NKYVLRIPSKDNKNNFSEENFVLIFANLNKLSPPIIYHNKDNGILISKFLEDSKVNMSTFTSLEFLEKLSINLRKLHILKCEH--IF-NPFEHIRKNFHILKSKNF-N---FHQ-------DIDLVL-NKLNILEEKLSKNMTIGLCHNDLNSSNVLYYN-------KNVLFIDFEFSAMCDIFFDLATVSW-M-LD------------------EKKRYFLIKSYFGYYS--------------Y-ELMEKLENYLFVVKLWNASWSFLKSLNT---NSTYDYKLGGNMIIDDLLSTL 5FTG Chain:A ((32-375)) ------FHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG----EAMVLESVMFAILAERSLGPKLYGIF--PQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLES---TPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRK-

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 888 -21431 -24.13 -85.72 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -24.13 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: